The Tecan Group and Oxford Nanopore Applied sciences plc (Oxford Nanopore) at the moment announce their collaboration to drive simpler high-throughput nanopore library preparation in an automatic, hands-free trend. The result’s a totally walkaway automated workflow for Oxford Nanopore’s Ligation Sequencing Package XL V14 protocol (LSK114) with Tecan’s DreamPrep® NGS options. The LSK114 protocol permits for any fragment size of DNA to be sequenced, which may vary from cell-free DNA to amplicons to any size DNA fragments, from 200 base pairs to greater than 100kb and even Mb. It is a main advance over conventional sequencing platforms and protocols that may solely sequence brief fragments sometimes within the vary of 300 to 600 base pairs.
Sequencing native, lengthy fragments of DNA is helpful to generate extra complete insights into the genome – which frequently comprise methylation, repetitive patterns of sequences or larger-scale genetic variants – in addition to reaching elements of the genome that can not be resolved through the use of conventional brief learn applied sciences in the identical single sequencing run. Having full genome assemblies is critical to make sure higher understanding of chromosome operate, human illness, and genomic variation, which is able to translate in future to higher prediction and administration of illnesses. Due to this fact, by combining extremely reproducible automated library preparation of brief and/or lengthy fragments and excessive yields of genomic knowledge, it turns into possible to reliably establish a complete map of genetic alterations, conduct correct phasing, resolve repeated sequences, and measure DNA methylation, all in a single sequencing run. Having the ability to deploy the identical workflow on shorter, native fragments, akin to cell-free DNA, opens alternatives in methylation evaluation and exploration of the entire cell-free DNA fragments in a pattern, the ‘fragmentome’.
At Tecan, we scale healthcare innovation, from life science to the clinic, and our partnership with Oxford Nanopore permits us to just do that. We carry push-button, walk-away comfort to high-throughput library preparation workflows, enabling scientists to concentrate on new discoveries as an alternative of tedious handbook work. Combining our experience in automation and high-quality library preparation with Oxford Nanopore sequencing and its capabilities round any-length reads, native DNA/RNA and actual time sequencing, has implications in a number of fields, from inhabitants genetics to most cancers analysis and customized drugs.”
Dr. Klaus Lun, Tecan Govt Vice President and Head of the Life Sciences Enterprise Division
Oxford Nanopore CEO Gordon Sanghera added, “Our objective at Oxford Nanopore is to ship reasonably priced and accessible plug-and-play platforms for organic analyses which can be presently carried out, and open new purposes which have a profound, constructive influence on enhancing our understanding of genetic alterations linked to illness. Our expertise now offers an automatic answer in a single platform, offering a complete genomic knowledge that features methylation, structural variation and replica quantity variation, in addition to commonplace SNP and SNV, calling for all organic questions together with methylation in the identical sequencing run. Our collaboration with Tecan is scaling accessible life sciences innovation with a worldwide accomplice, offering automated seamless plug-and-play options to our clients in faster, larger high quality outcomes that save time, price and energy.”
The collaboration between Tecan and Oxford Nanopore additional expands Tecan’s rising portfolio of options for automated NGS library preparation. Tecan’s liquid handlers are suitable with all Oxford Nanopore gadgets – from Flongle™ to PromethION™ 48, in addition to the PromethION 2 (P2) Solo high-output sequencing machine. This provides the potential to drastically broaden entry to correct, quick and accessible sequencing for human genomics and different bigger datasets.
Oxford Nanopore’s expertise permits base-modification evaluation to be carried out alongside nucleotide sequencing on the identical single learn with out the necessity to run a number of sequencing Tecan Group Ltd., Seestrasse 103, CH-8708 Männedorf, Switzerland T +41 44 922 88 88, F +41 44 922 88 89, [email protected], www.tecan.com 2 experiments. Due to this fact, in contrast to conventional applied sciences, no further advanced library preparation with (for instance bisulfite sequencing for methylation) is required, and epigenetic modification evaluation could be carried out throughout the entire genome throughout the experiment. Tecan automated library preparation methods rework time-consuming and error-prone procedures into easy, strong consumer experiences. The built-in mixture of Oxford Nanopore and Tecan applied sciences is meant to boost true load-and-go DNA and RNA sequencing library preparation with confirmed liquid dealing with expertise, reagent kits which can be suitable with automation, and intuitive consumer interfaces that can permit set-up for walkaway library prep runs within the DreamPrep NGS.
Protocol Ligation Sequencing Package and DreamPrep Technical Observe
A technical be aware launched at the moment is proof of idea and demonstrates the velocity, accessibility, and automatic consistency the collaboration delivers for a wide range of purposes. The be aware reveals a consumer case the place the Oxford Nanopore’s Ligation Sequencing Package XL V14 processing a number of DNA samples on the Tecan DreamPrep NGS achieves excessive yield and uncooked learn sequencing modal accuracy of Q20 (99%) and above. Attendees of the Society for Laboratory Automation and Screening (SLAS) Worldwide Convention and Exhibition (San Diego, February 25-March 1, 2023) will see a preview of this knowledge and may have a possibility to debate the targets of each corporations of their joint efforts to create protocols permitting for extra scalable and better throughput sequencing.
