Renishaw is happy to introduce fluorescence lifetime imaging microscopy (FLIM) performance to its inVia™ confocal Raman microscope. In partnership with Becker & Hickl GmbH, the pioneers in time-correlated single photon counting (TCSPC), the system combines each Raman and FLIM in a single instrument. This integration can overlay each FLIM and Raman photographs with pixel-to-pixel correlation, thus growing understanding of samples throughout a spread of functions.
Picture Credit score: Renishaw
FLIM imaging
Within the system, when mild from a pulsed laser interacts with a fluorophore, the fluorescence lifetime is measured utilizing a complicated detector and high-speed electronics. This fluorescence lifetime is the attribute time a fluorophore stays in an excited digital state earlier than returning to the bottom state by emitting a photon. The lifetime of fluorescence decay is delicate to the molecular surroundings and the ensuing adjustments in molecular conformation. The FLIM approach can be utilized to check a spread of properties from pH/ion/oxygen focus to molecular binding.
Mixed FLIM with Raman microscopy
FLIM and Raman microscopy are complementary strategies for label-free imaging of organic cells and tissue sections. FLIM supplies perception into molecular interactions and could be carried out at the very least 10× quicker than Raman imaging. Raman spectroscopy boasts excessive chemical specificity and high-resolution chemical photographs. This mixture of strategies is good for fast identification of areas of curiosity by FLIM, adopted by chemical and structural characterisation by Raman imaging.
For FLIM knowledge evaluation, complete software program from Becker & Hickl is included with multi-exponential, incomplete and shifted-component evaluation fashions. The software program additionally contains proprietary phasor evaluation to differentiate lifetime populations. Fluorescence lifetime and Raman photographs could be instantly overlaid as a result of the usage of the extremely correct Renishaw MS30 excessive velocity encoded stage for each imaging modalities.
“We’re happy to announce an additional enhancement to our award-winning inVia Raman microscope. Our collaboration with the leaders in FLIM know-how, Becker and Hickl, has produced a mixed FLIM Raman microscope resulting in thrilling new utility areas.”
David Reece, Renishaw Spectroscopy Enterprise Improvement Supervisor
For additional data on fluorescence lifetime imaging microscopy (FLIM): Fluorescence lifetime imaging microscopy (FLIM) (renishaw.com)
For additional data on Renishaw Raman Spectroscopy: www.renishaw.com/raman
